Scientific publications

Membrane protein profiling of human islets of Langerhans using several extraction methods

Clin. Proteom., 2010, 6(4), 195 - 207
Sara F. Hansson, Ã…sa Henriksson, Lars Johansson, Olle Korsgren, Jan W. Eriksson, Hans Tornqvist, Pia Davidsson

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Improving the depth of coverage in membrane proteomic studies through the use of lipid-based protein immobilization technology in parallel with methanol-facilitated solubilisation

Anal. Methods, 2010, 2, 539 - 545
Neerav D. Padliya, Mohit B. Bhatia, Wolfgang T. Hofgärtner and Robert J. Hariri

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Elucidation of the outer membrane proteome of Salmonella enterica serovar Typhimurium utilising a lipid-based protein immobilization technique

BMC Microbiol., 2010, 10, 44
Darren Chooneea, Roger Karlsson, Vesela Encheva, Cath Arnold, Hazel Appleton, and Haroun Shah

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Identification of key proteins involved in the anammox reaction

FEMS Microbiology Letters, 2009, 297(1), 87 - 94
Roger Karlsson, Anders Karlsson, Ola Bäckman, Bengt R. Johansson, Stefan Hulth

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Proteomic analysis of plasma membrane vesicles

Angew. Chem. Int. Ed. Engl., 2009, 48(9), 1656 - 1659
Bauer B, Davidson M, Orwar O

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FEMS Microbiology Letters, 2009, 297(1), 87-94

Bacteria performing anaerobic ammonium oxidation (anammox) are key players in the global nitrogen cycle due to their inherent ability to convert biologically available nitrogen to N2. Anammox is increasingly being exploited during wastewater treatment worldwide, and about 50% of the total N2 production in marine environments is estimated to proceed by the anammox pathway. To fully understand the microbial functionality and mechanisms that control environmental feedbacks of the anammox reaction, key proteins involved in the reaction must be identified. In this study we have utilized an analytical protocol that facilitates detection of proteins associated with the anammoxosome, an intracellular membrane compartment within the anammox bacterium. The protocol enabled us to identify several key proteins of the anammox reaction including a hydrazine hydrolase producing hydrazine, a hydrazine-oxidizing enzyme converting hydrazine to N2 and a membrane-bound ATP synthase generating ATP from the gradients of protons formed in the anammox reaction. We also performed immunogold labelling electron microscopy to determine the subcellular location of the hydrazine hydrolase. The results from our study support the hypothesis that proteins associated with the anammoxosome host the complete suite of reactions during anammox.