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Scientific publications
 
 
 

Membrane protein profiling of human islets of Langerhans using several extraction methods

Clin. Proteom., 2010, 6(4), 195 - 207
Sara F. Hansson, Ã…sa Henriksson, Lars Johansson, Olle Korsgren, Jan W. Eriksson, Hans Tornqvist, Pia Davidsson

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Improving the depth of coverage in membrane proteomic studies through the use of lipid-based protein immobilization technology in parallel with methanol-facilitated solubilisation

Anal. Methods, 2010, 2, 539 - 545
Neerav D. Padliya, Mohit B. Bhatia, Wolfgang T. Hofgärtner and Robert J. Hariri

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Elucidation of the outer membrane proteome of Salmonella enterica serovar Typhimurium utilising a lipid-based protein immobilization technique

BMC Microbiol., 2010, 10, 44
Darren Chooneea, Roger Karlsson, Vesela Encheva, Cath Arnold, Hazel Appleton, and Haroun Shah

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Identification of key proteins involved in the anammox reaction

FEMS Microbiology Letters, 2009, 297(1), 87 - 94
Roger Karlsson, Anders Karlsson, Ola Bäckman, Bengt R. Johansson, Stefan Hulth

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Proteomic analysis of plasma membrane vesicles

Angew. Chem. Int. Ed. Engl., 2009, 48(9), 1656 - 1659
Bauer B, Davidson M, Orwar O

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BMC Microbiol., 2010, 10, 44

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major cause of human gastroenteritis worldwide. The outer membrane proteins expressed by S. Typhimurium mediate the process of adhesion and internalisation within the intestinal epithelium of the host thus influencing the progression of disease. Since the outer membrane proteins are surface-exposed, they provide attractive targets for the development of improved antimicrobial agents and vaccines. Various techniques have been developed for their characterisation, but issues such as carryover of cytosolic proteins still remain a problem. In this study we attempted to characterise the surface proteome of S. Typhimurium using Lipid-based Protein Immobilisation technology in the form of LPITM FlowCells. No detergents are required and no sample clean up is needed prior to downstream analysis. The immobilised proteins can be digested with proteases in multiple steps to increase sequence coverage, and the peptides eluted can be characterised directly by liquid chromatography - tandem mass spectrometry (LC-MS/MS) and identified from mass spectral database searches.